Leica SP5 Confocal/MultiPhoton with Resonant Scanner

What it’s good for

- Determining colocalization for multiple signals
- Acquisition of up to 3 color channels simultaneously
- Acquisition of multiple color channels sequentially
- Acquisition of a transmitted light image
- Fixed cells or tissues on a slide with a coverslip (#1.5) or in a dish
- Live tissues or Live Animals
- Spectral Deconvolution to correct for overlapping signals
- Calcium Dynamics - Fast Scanning with Resonant Scanner
- FRET
- Stimulus applications
- FRAP
- FLIP
- Photoactivation
- Photoconversion
- Uncaging

What it’s not good for

- Live Cells: Can be very harsh for some live cell applications and an upright system is not ideal for live cells in a dish

Principles of operation

Laser light of specific wavelengths is scanned across the sample and filtered before detection to produce a high resolution image composed of a small optical slice of the sample.

Technical Information

- Microscope: Leica Upright
- Incubated with CO2
- Spectral for all channels
laser lines
- 405
- 458
- 488
- 515
- 543
- 594
- 633
- Mai Tai HP 2-photon laser tunable from 720nm - 1040nm

Microscope objectives
- 10x (.40 NA)
- 20x (.75 NA)
- 63x Glycerol (1.30 NA)
- 20x Water (1.00 NA)
- 40x Water (.8 NA)

Leica LAS Lite Software Download



 
 
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