Olympus FV1000 Confocal

What it's good for

- Determining colocalization for multiple signals
- Acquisition of up to 4 color channels simultaneously
- Acquisition of up to 16 color channels sequentially
- Acquisition of a high contrast DIC image
- Fixed cells or tissues on a slide with a coverslip (#1.5)
- Live cells or tissues in a chamber or dish with a coverslip bottom (#1.5)
- Spectral Deconvolution to correct for overlapping signals
- Multipoint timelapse and panel acquisition
- Calcium Dynamics
- FRET
- Stimulus applications 
- FRAP
- FLIP
- Photoactivation
- Photoconversion
- Uncaging

What it's not good for

- Dim signal
- Thick tissues (>50 micons)
- Live Cells: Can be very harsh for some live cell applications

Principles of operation

Laser light of specific wavelengths is scanned across the sample and filtered before detection to produce a high resolution image composed of a small optical slice of the sample.

Technical Information

- Microscope: Olympus IX81 inverted
- Incubated with CO2
- DIC channel
- 6 laser lines
- 405
- 458
- 488
- 515
- 543
- 633
- Microscope objectives
- 4x (.13 NA)
- 10x (.40 NA)
- 20x (.75 NA)
- 40x (1.30 NA)
- 60x (1.42 NA)
- 100x (1.40 NA)

FluoView Viewer Software Download (serial number 8L62)


Room 008C


 
 
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