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Events & Updates

New Microscopy Core Resources

 Zeiss Elyra 7 with Lattice SIM

  • Resolve structures down to 60 nm.
  • Observe live cell dynamics at up to 255 fps.
  • Accelerate image acquisition in all three dimensions.
  • Get the sharpest sectioning in wide-field microscopy.
  • Utilize a wealth of imaging techniques on one platform.

Contact us today for a free demonstration: jsantini@health.ucsd.edu

Elyra 7 SIM resolution

Olympus VS200 Slide Scanner

The sliding scanning system is superior in fluorescence and brightfield capabilities

Leica Center of Excellence

The UC San Diego School of Medicine Microscopy Core is now a Leica Center of Excellence! We have three Leica systems that are supported by Leica:

Leica Representative Onsite

A Leica representative can come onsite for assistance as requested

Core staff are available during business hours to answer any questions regarding all core resources and techniques


Webinars, Seminars and Demonstrations

Informational Webinar

Visualizing membrane transformation in Live Cell with STED: Fusion and Budding

Ling-Gang Wu Ph.D.
Senior Investigator, Synaptic Transmission Section, NINDS NIH
EVENT DATE & TIME
Date: June 4, 2024
Time: 9:00 AM (PDT), 12:00 PM (EDT), 6:00 PM (CEST)
ABSTRACT
Membrane fusion and budding mediate fundamental biological processes like intracellular trafficking, exocytosis, and endocytosis. For many decades, fusion has been thought to open a nanometer-range pore that may subsequently close or dilate irreversibly, whereas budding transforms flat membranes into vesicles. This classical view has been difficult to verify owing to the difficulty of seeing membrane transformation in real time.
 
In this webinar, Dr. Wu will present a newly developed workflow to visualize in real-time the membrane transformations of exo- and endocytosis in live cells (neuroendocrine chromaffin cells). Real-time visualization reveals membrane transformations of exo- and endocytosis far exceeding the classical view. He synthesizes a new model of exo- and endocytosis based on real-time observations and describes its underlying mechanistic principles and functions.
 
In this new model, fusion involves hemi-to-full fusion, pore expansion, constriction and/or closure while fusing vesicles may shrink, enlarge, or receive another vesicle fusion; endocytosis follows exocytosis primarily by closing W-shaped profiles pre-formed through the flat-to-L-to-W-shape transition or formed via fusion. Calcium/SNARE-dependent fusion machinery, cytoskeleton-dependent membrane tension, osmotic pressure, calcium/dynamin-dependent fission machinery, and actin/dynamin-dependent force machinery work together to generate fusion and budding modes differing in pore status, vesicle size, speed and quantity, controls release probability, synchronization, and content release rates/amounts, and underlies exo-endocytosis coupling to maintain membrane homeostasis. These transformations, underlying molecular mechanics, and functions may be conserved for fusion and budding in general.
 
Learning Objectives
  • Examine exocytotic vesicle membrane and fusion pore dynamics and their underlying mechanics.
  • Demonstrate endocytic transformation from flat to L-, W-, and O-shape and underlying mechanics.
  • Discuss a workflow for visualizing vesicle fusion and budding in real-time.

Registration

Microscopy Seminar Series

UC San Diego School of Medicine Microscopy Seminar Series will be cancelled until further notice. We will reschedule seminars once concerns are lessened.

Citations

All publications generated utilizing our resources must cite our grant:

NINDS P30NS047101

If the Elyra 7 Lattice SIM Instrument was utilized, please cite our S10 grant:

S10OD030505